Nov. 5, 2018
9:30 – 11:30 AM
NIH Bldg 10, FAES classroom 3, B1C207
Presenter: Peter Fitzgerald, Ph.D., CCR Genome Analysis Unit
This introductory lecture will provide an overview of RNA-Seq technology, its various application and shortcomings, as well as detailing the steps involved in analyzing the resulting data.
The specific topics covered include:
- What is RNA-Seq?
- What can it be used for?
- Sequencing platforms
- Quality Control steps
- Experimental Design
- Data Analysis Workflows
- Identifying differentially expressed gene
- Discussion of relevant file formats and data conversion tools
- Approaches to detect splice variants and fusion genes
- NCI specific tools and software