User Profile kellymc@nih.govQuestions(9)Answers(16)What are the Rules and Purpose of this ForumClosedfitzgepe@nih.gov answered 2 years ago • BIOSTAR4398 views1 answers0 votesFor single nuclei RNA-Seq, do I have to do anything different in handling the data?Answereddadkhahe@nih.gov answered 2 years ago • Single-Cell RNA-Seq1489 views1 answers0 votesDifference between 3′ and 5′ gene expression profiling? Which should I use?Answereddadkhahe@nih.gov answered 2 years ago • Single-Cell RNA-Seq7890 views2 answers0 votesDroplet-based (e.g. 10x Genomics) or plated-based (e.g. Smart-Seq)? When should one be considered over the otherAnswereddadkhahe@nih.gov answered 2 years ago • Single-Cell RNA-Seq4666 views1 answers0 votesWhat sequencing depth do I need for scRNA-Seq samples?Answereddadkhahe@nih.gov answered 2 years ago • Single-Cell RNA-Seq2814 views2 answers0 votesHow do we decide what clustering resolution to set?Openkellymc@nih.gov asked 2 years ago • Single-Cell RNA-Seq1392 views0 answers0 votesAre there standard pre-processing criteria that should be used?Openkellymc@nih.gov asked 2 years ago • Single-Cell RNA-Seq1244 views0 answers0 votesWhat are the minimal number of cells for robust single cell RNA-Seq data?Openkellymc@nih.gov asked 2 years ago • Single-Cell RNA-Seq1362 views0 answers0 votesWhen should we batch correct scRNA-Seq data? When should we avoid it?Answeredwongnw@nih.gov answered 2 years ago • Single-Cell RNA-Seq10715 views2 answers0 votesFull length transcripts from single cell (even droplet-based)?Openkellymc@nih.gov asked 2 years ago • Single-Cell RNA-Seq1363 views0 answers0 votesCrop