QuestionsCategory: Single-Cell RNA-SeqWhich cell types can be used in Single-Cell RNA Seq? asked 3 years ago
1 Answers answered 3 years ago

Short answer is that with any oligo-dT based single cell RNA-seq approach, any cell that has poly-adenylate transcripts and can be efficiently partitioned and lysed can be used for single cell RNA-Seq.
Some new methods allow total RNA: and some commercial solutions do not rely on oligo-dT based reverse transcription priming, which opens up several other possible applications: