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Questions › Category: Single-Cell RNA-Seq
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Why don’t the scRNA transcript counts match up with the results I found with flow cytometry?
Answeredkellymc@nih.gov answered 1 year ago • 
869 views1 answers0 votes
For single nuclei RNA-Seq, do I have to do anything different in handling the data?
Answereddadkhahe@nih.gov answered 1 year ago • 
721 views1 answers0 votes
Difference between 3′ and 5′ gene expression profiling? Which should I use?
Answereddadkhahe@nih.gov answered 1 year ago • 
4029 views2 answers0 votes
What is Cell Ranger?
Answereddadkhahe@nih.gov answered 1 year ago • 
777 views1 answers0 votes
Do I need to have extensive bioinformatic knowledge to explore my Cell Ranger results?
Answereddadkhahe@nih.gov answered 1 year ago • 
697 views1 answers0 votes
Droplet-based (e.g. 10x Genomics) or plated-based (e.g. Smart-Seq)? When should one be considered over the other
Answereddadkhahe@nih.gov answered 1 year ago • 
2240 views1 answers0 votes
What sequencing depth do I need for scRNA-Seq samples?
Answereddadkhahe@nih.gov answered 1 year ago • 
1264 views2 answers0 votes
How do we decide what clustering resolution to set?
Openkellymc@nih.gov asked 1 year ago • 
634 views0 answers0 votes
Are there standard pre-processing criteria that should be used?
Openkellymc@nih.gov asked 1 year ago • 
634 views0 answers0 votes
What are the minimal number of cells for robust single cell RNA-Seq data?
Openkellymc@nih.gov asked 1 year ago • 
688 views0 answers0 votes
Can you give some suggestions cell identity annoation and recommend packages good at cell identity annotation? What is the most used practice, manually or auto?
Answeredkellymc@nih.gov answered 1 year ago • 
1375 views2 answers0 votes
Can you say something about trajectory analysis vs. velocity analysis?
Answeredkellymc@nih.gov answered 1 year ago • 
3290 views2 answers0 votes
How can I use a large data set like Tabula Muris for identifying cell types in my data?
Answeredoguzc@nih.gov answered 1 year ago • 
1045 views1 answers0 votes
When should we batch correct scRNA-Seq data? When should we avoid it?
Answeredwongnw@nih.gov answered 1 year ago • 
4598 views2 answers0 votes
Full length transcripts from single cell (even droplet-based)?
Openkellymc@nih.gov asked 1 year ago • 
654 views0 answers0 votes
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