Questions › Category: ChIP-Seq Data Analysis › Spike-ins: At what stage of the ChIP experiment do you introduce the spike-ins?
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stonelakeak@nih.gov Staff asked 8 months ago

At what stage of the ChIP experiment do you introduce the spike-ins?

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stonelakeak@nih.gov Staff answered 8 months ago

There are a few different stages when you can add the spike-ins. The most important thing is to make sure they are combined prior to the pull-down step. This includes combining cells together, combining spheroplasts together, combining nuclei together, or combining fragmented DNA together. by Tovah Markowitz, Paul Schaughency, Vishal Koparde.