QuestionsCategory: ChIP-Seq Data AnalysisSpike-ins: At what stage of the ChIP experiment do you introduce the spike-ins?
stonelakeak@nih.gov Staff asked 5 months ago

At what stage of the ChIP experiment do you introduce the spike-ins?

1 Answers
stonelakeak@nih.gov Staff answered 5 months ago

There are a few different stages when you can add the spike-ins. The most important thing is to make sure they are combined prior to the pull-down step. This includes combining cells together, combining spheroplasts together, combining nuclei together, or combining fragmented DNA together. by Tovah Markowitz, Paul Schaughency, Vishal Koparde.