This will really depend on a few empirically derived factors (antibody binding affinity for example). In general, you want to start with a much DNA as possible for each sample whether it is fresh or fixed. The NEBNext ChIP-seq Library Prep Kit recommends 1ng-10ng minimum AFTER immunoprecipitation. Citation:
– answered by Tovah Markowitz, Paul Schaughency, Vishal Koparde.
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