QuestionsCategory: ChIP-Seq Data AnalysisLibrary ChIP preparation: What happens if there are too many PCR cycles?
stonelakeak@nih.gov Staff asked 5 months ago

What happens if there are too many PCR cycles?

1 Answers
stonelakeak@nih.gov Staff answered 5 months ago

You get a lot of duplicated reads downstream in the analysis which will decrease the amount of information you get from your ChIP-seq. You should be able to detect this in the lab prior to sequencing as a random super sharp band on your gel, Bioanalyzer, or TapeStation. – answered by Tovah Markowitz, Paul Schaughency, Vishal Koparde.