QuestionsCategory: ChIP-Seq Data AnalysisLibrary/ChIP preparation: Is it okay to use frozen samples or is it better to use fresh cells?
stonelakeak@nih.gov Staff asked 5 months ago

Is it okay to use frozen samples or is it better to use fresh cells?

1 Answers
stonelakeak@nih.gov Staff answered 5 months ago

There should be no appreciable difference between using frozen vs fresh cells. That being said, since the ChIP method uses fixation to stabilize the DNA-protein interactions, it is advisable to first fix the cells for long term storage. There is the possibility that freeze thaw of frozen tissues or cells may alter interactions. – answered by Tovah Markowitz, Paul Schaughency, Vishal Koparde.