QuestionsCategory: ChIP-Seq Data AnalysisLibrary ChIP preparation: Can ChIP-seq be done on formalin fixed tissue and if yes how much starting material is usually required?
stonelakeak@nih.gov Staff asked 5 months ago

Can ChIP-seq be done on formalin fixed tissue and if yes how much starting material is usually required?

1 Answers
stonelakeak@nih.gov Staff answered 5 months ago

Formalin fixed tissues should be able to be used for ChIP-seq granted that you can get enough DNA out of the extraction protocol. Typically, ChIP uses formaldehyde crosslinking to preserve the DNA-protein interactions. Since these tissues are already fixed, once extracted from the paraffin, the DNA should be able to be fragmented and taken through the rest of the protocol. Citation: https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5639-8 – answered by Tovah Markowitz, Paul Schaughency, Vishal Koparde.