Nov. 6, 2018
9:30 AM – 12 PM
THIS CLASS HAS BEEN MOVED TO BLDG 37, RM 6041/6107
Please bring a laptop or let us know if you need to borrow one. Please bring handout downloaded from class website -> BTEP/Training/Upcoming classes
Speaker: Xiaowen Wang, Partek
RNA-Seq Data Analysis in Partek Flow
During this hands on training session, students will learn how to use the point-and-click interface in Partek Flow to go from raw data to experimental results for RNA-Seq analysis:
– Import data from .fastq files
– Perform QA/QC (Pre-alignment QA/QC, Post-alignment QA/QC)
– Trim bases
– Align reads to reference genome
– Quantify gene/transcript abundance
– Normalize gene counts
– Detect differentially expressed genes
– Filter a gene list
– Identify enriched KEGG pathway and/or GO terms
– Visualization:
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- Heat maps
- Volcano plots
- PCA scatterplot
- Dot plots
- Hierarchical clustering
- Chromosome view
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