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Bioinformatics Training and Education Program

november, 2020

05nov1:00 pm2:00 pmSpatial Transcriptomic Analysis and Cell Type Characterization Using the RNAscope HiPlex AssayJoin us for an ACD | NIH Spatial Genomics Webinar


Event Details


Characterizing the transcriptomic profiles of individual cells by single-cell
RNA sequencing (scRNA-seq) has become a universal tool to identify
both known and novel cell populations, ushering science in a new era of
single cell biology. However, scRNA-seq utilizes dissociated cells with
results in the loss of spatial organization of the cell population being
It is therefore essential to complement scRNA-seq analysis with
RNAscope in situ hybridization (ISH) in order to obtain visual
confirmation of both single cell and spatial gene expression.
In this webinar, Dr. Ariel Levine from NIH NINDS will share her latest
publication using RNAscope HiPlex assay, to reveal spinal cord cell type
organization, validate a combinatorial set of markers for in-tissue spatial
gene expression analysis, and optimize the computational classification

Presented by:
Ariel Levine, Ph.D.
Earl Stadtman Investigator
National Institute of Neurological Disorders and Stroke


(Thursday) 1:00 pm - 2:00 pm