This training will provide an introduction to RNA-seq data analysis followed by tutorials showing the use of popular RNA-seq analysis packages and preparing participants to independently run basic RNA-Seq analysis for expression profiling. The exercise will run on the Galaxy platform using Illumina paired-end RNA-seq data.

Participants will have a chance to: run quality control check on sequencing data; align the sequencing reads to a reference genome; generate alignment statistics and check mapping quality; measure abundance of transcripts; perform differential expression analysis; and visualize the output of RNA-seq analyses.