This introductory lecture will provide an overview of RNA-Seq technology, its various application and shortcomings, as well as detailing the steps involved in analyzing the resulting data.
The specific topics covered include:
- What is RNASeq ?
- What can it be used for ?
- Sequencing platforms
- Quality Control steps
- Experimental Design
- Data Analysis Workflows
- Identifying differentially expressed genes
- Discussion of relevant file formats and data conversion tools
- Approaches to detect splice variants and fusion genes
- NCI specific tools and software
Followed by: A Practical Guide to Interpreting RNA-Seq Data
Next-generation sequencing (NGS) RNA-seq experiments generate millions of reads per run and problems can arise. Before analyzing the data to draw biological conclusions, it is important to perform quality control checks to minimize the effects of sequencing error, biases in the data, or contamination. In this talk, we discuss the theory and practice of assessing the quality of RNA-Seq data, as well as analysis strategies for mitigating technical noise using practical examples.
If you are unable to attend in person, the workshop will be available via WebEx and recorded.
BTEP, RNA-Seq Workshop: Intro to RNA-Seq Technology, Overview and Analyses
Hosted by Amy Stonelake
Tuesday, May 21, 2019 2:00 pm | 3 hours | (UTC-05:00) Eastern Time (US & Canada)
Meeting number: 731 553 624
Join by video system
You can also dial 188.8.131.52 and enter your meeting number.
Join by phone
1-650-479-3207 Call-in toll number (US/Canada)
Access code: 731 553 624
Link to WebEx recording:
Streaming recording link:
Download recording link:
Course Material 1: BTEP_RNASeq_Final_2019.pdf
Course Material 2: RNA-seq_BETP_2019rev.pdf