Course Details

  • Date: August 28th, 2019 - August 28th, 2019
  • Time: 9:00 am - 11:00 am
  • Location: Building 37, Room 4041/4107
  • Presenter(s): Xiaowen Wang (Partek)

Please bring your computer with Partek Genomics Suite to this hands-on workshop. You’ll need to submit a request through “NCI at Your Service” to obtain the access to this software.

Partek will demonstrate both Microarray and Methylation Data Analysis in Partek Genomics Suite with Pathway

***Microarray Data Analysis in Partek Genomics Suite with Pathway***

Abstract: The class will start with an overview of Partek Genomics Suite with Pathway and followed by a hands-on tutorial session using a sample Gene Expression dataset.  The hands-on session will help attendees gain expertise on the following topics: data import, QA/QC, exploratory data analysis, differential expression analysis, visualizations, and biological interpretation as well as other basic analysis in Partek Genomics Suite with Pathway.

  • Data Import
  • QA/QC
  • Exploratory Data Analysis
  • Differential Expression Analysis
  • Visualization (PCA, hierarchical clustering, dot plot, chromosome view, volcano plot, etc.)
  • Biological Interpretation

***Microarray Methylation Data Analysis in Partek Genomics Suite***

The class will use the Illumina 450K Methylation array data. The workflow can also be used on HumanMethylation27 (27K) and HumanMethylationEPIC (850K) BeadChips. Attendees will learn how to perform QA/QC, detect differential methylation, find genes overlapping CpG loci and biological interpretation in Partek Genomics Suite with Pathway.

– Import data from Illumina methylation array in .idat files

–  Methylation array-specific normalization

–  Perform QA/AC

–  Detection of differentially methylated CpG loci

–  Creating list of loci of interest

  • Identifying methylation signatures
  • Find overlapping genes
  • Biological interpretation
  • Visualization
    • PCA
    • Dot plot
    • Hierarchical clustering
    • Pathway

If you can not attend in person, WebEx will be provided.

Meeting number (access code): 733 186 710
Meeting password: JdyNKF@3
Wednesday, August 28, 2019
8:30 am  |  (UTC-05:00) Eastern Time (US & Canada)  |  2 hrs 30 mins
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