Course Details

  • Date: October 23rd, 2019 - October 23rd, 2019
  • Time: 1:30 pm - 3:30 pm
  • Location: Bldg 10 FAES room 4 (B1C205)
  • Presenter(s): Xiaowen Wang (Partek)

Please note class will start at 1:30 PM and end at 3:30 PM. 

Hands On Training on Single Cell RNA-Seq Data Analysis in Partek Flow

Please bring your own computer for this hands-on training. If you need to borrow a computer please email ncibtep@nih.gov

Abstract: Students will learn how to identify cell populations and detect differentially expressed genes in a simple Single Cell RNA-Seq experiment with the point-and-click interface in Partek Flow:

– Import data

– Filter cells using interactive QA/QC charts

– Filter and normalize Single Cell RNA-Seq data

– Visualize cell populations using the interactive 3D t-SNE plot

– Overlay gene expression and pathway signatures on the 3D t-SNE plot

– Select and classify cells on the 3D t-SNE plot

– Detect differentially expressed genes

– Filter a gene list

– Identify enriched KEGG pathway and/or GO terms

– Visualize cell-level results using heat maps, volcano plots, and violin plots

If you are unable to attend in person WebEx will be available:

Meeting link:
https://cbiit.webex.com/cbiit/j.php?MTID=m93cd8514270a1242d230b21a8379fb2f
Meeting number: 737 711 601
Password: Cxjjjq@3
Host key: 696480
More ways to join
Join by video system
Dial 737711601@cbiit.webex.com
You can also dial 173.243.2.68 and enter your meeting number.
Join by phone
1-650-479-3207 Call-in number (US/Canada)
Access code: 737 711 601

Course Material 1: PDF icon PartekFlowNewFeatureList_AUG_2019.pdf

Course Material 2: PDF icon PartekFlowSingleCellRNA-SeqTrainingHandout_August2019-1.pdf