Course Details

  • Date: May 10th, 2019 - May 10th, 2019
  • Time: 9:30 am - 12:30 pm
  • Location: NIH Bldg 37, Rm 2041/2107
  • Presenter(s): Alexei Lobanov (CCBR), Paul Schaughency (NCBR), Tovah Markowitz (NCBR)

This workshop will teach the basic concepts and practical aspects of data analysis. Topics covered will include: experimental approach, quality control, peak calling, and basic biological interpretation. No computer is required for this class, there is no hands-on work.

9:30-10:15 “ChIP-seq considerations”

This section will focus on the importance of experimental design in ChIP-seq. Topics include: variations on ChIP-seq, inputs, replicates, the limitations of ChIP-seq, and the value of follow-up analyses.

10:15-10:30 Break

10:30-12:00 “Analysis of ChIP-seq data

This section will focus on the analytical pipeline that is implemented by CCBR and NCBR. Time will be spent discussing the tools used, why they were chosen, and how to make sense of their outputs. Example results will be shown. Topics include: quality control and read alignment, deduplication, normalization, gene coverage plotting, peak calling, motif analysis, and more.

12:00-12:30 Questions

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If you are unable to attend in person, WebEx will be provided. A recording of the meeting will be made available on the BTEP web site.

ChIP-Seq Data Analysis: Probing DNA-Protein Interactions

Hosted by Amy Stonelake

Friday, May 10, 2019 9:30 am | 3 hours | (UTC-05:00) Eastern Time (US & Canada)

Meeting number: 737 294 613

Password: JwrMe6p@

https://cbiit.webex.com/cbiit/j.php?MTID=m4b85110c7a550bd3815d04ae184f6e1b

 

Join by video system

Dial 737294613@cbiit.webex.com

You can also dial 173.243.2.68 and enter your meeting number.

 

Join by phone

1-650-479-3207 Call-in toll number (US/Canada)

Access code: 737 294 613

Course Material 1: PDF icon BTEP_ChIP_May2019_Part1.pdf

Course Material 2: PDF icon BTEP_ChIP_May2019_Part2.pdf


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