This workshop will teach the basic concepts and practical aspects of data analysis. Topics covered will include: experimental approach, quality control, peak calling, and basic biological interpretation. No computer is required for this class, there is no hands-on work.
9:30-10:15 “ChIP-seq considerations”
This section will focus on the importance of experimental design in ChIP-seq. Topics include: variations on ChIP-seq, inputs, replicates, the limitations of ChIP-seq, and the value of follow-up analyses.
10:15-10:30 Break
10:30-12:00 “Analysis of ChIP-seq data
This section will focus on the analytical pipeline that is implemented by CCBR and NCBR. Time will be spent discussing the tools used, why they were chosen, and how to make sense of their outputs. Example results will be shown. Topics include: quality control and read alignment, deduplication, normalization, gene coverage plotting, peak calling, motif analysis, and more.
12:00-12:30 Questions
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If you are unable to attend in person, WebEx will be provided. A recording of the meeting will be made available on the BTEP web site.
ChIP-Seq Data Analysis: Probing DNA-Protein Interactions
Hosted by Amy Stonelake
Friday, May 10, 2019 9:30 am | 3 hours | (UTC-05:00) Eastern Time (US & Canada)
Meeting number: 737 294 613
Password: JwrMe6p@
https://cbiit.webex.com/cbiit/j.php?MTID=m4b85110c7a550bd3815d04ae184f6e1b
Join by video system
Dial 737294613@cbiit.webex.com
You can also dial 173.243.2.68 and enter your meeting number.
Join by phone
1-650-479-3207 Call-in toll number (US/Canada)
Access code: 737 294 613
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