RNA sequencing (RNA-Seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the Palantir collaboration platform, which is available and free to use for all NCI researchers. The platform allows users to explore and build their analyses in a graphical environment. For researchers who are conversant in code, or who are interested in learning, all of the workflow code is easily viewable and modifiable, as well.
This training session will include a brief lecture on the theory behind RNA-seq. Most of the class time will be devoted to a workshop in which trainees will be guided through a basic analysis of a real RNA-seq dataset. Topics you will learn about include filtering and QC, PCA, expression heatmaps, differential expression, volcano plots, differential expression of genes analysis, and pathway/gene set analysis. After training, trainees will be ready to upload their own data to the platform and begin their own analyses.
Trainees will need an NIH laptop capable of connecting to the secure NIH wireless network, as well as their own NIH credentials.